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INTRODUCTION
From last two decades nanotechnology played an important role in the industrial revolution. Nano particles have at least one dimention with size 1 to 100nm. ADDIN EN.CITE <EndNote><Cite><Author>Fabrega</Author><Year>2011</Year><RecNum>6</RecNum><DisplayText>(Fabrega, Luoma, Tyler, Galloway, &amp; Lead, 2011)</DisplayText><record><rec-number>6</rec-number><foreign-keys><key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”>6</key></foreign-keys><ref-type name=”Journal Article”>17</ref-type><contributors><authors><author>Fabrega, Julia</author><author>Luoma, Samuel N</author><author>Tyler, Charles R</author><author>Galloway, Tamara S</author><author>Lead, Jamie R</author></authors></contributors><titles><title>Silver nanoparticles: behaviour and effects in the aquatic environment</title><secondary-title>Environment international</secondary-title></titles><periodical><full-title>Environment international</full-title></periodical><pages>517-531</pages><volume>37</volume><number>2</number><dates><year>2011</year></dates><isbn>0160-4120</isbn><urls></urls></record></Cite></EndNote>(Fabrega, Luoma, Tyler, Galloway, & Lead, 2011) Due to their optical, mechanical, electrical, chemical applications NPs are used most widely in every field now a days. ADDIN EN.CITE <EndNote><Cite><Author>Yaghmour</Author><Year>2013</Year><RecNum>4</RecNum><DisplayText>(Yaghmour &amp; Mahmoud, 2013)</DisplayText><record><rec-number>4</rec-number><foreign-keys><key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”>4</key></foreign-keys><ref-type name=”Journal Article”>17</ref-type><contributors><authors><author>Yaghmour, SJ</author><author>Mahmoud, Waleed E</author></authors></contributors><titles><title>Synthesis and characterization of self-assembly silver sulfide nanorods prepared by squalene assisted microwave technique</title><secondary-title>Materials Letters</secondary-title></titles><periodical><full-title>Materials Letters</full-title></periodical><pages>55-57</pages><volume>109</volume><dates><year>2013</year></dates><isbn>0167-577X</isbn><urls></urls></record></Cite></EndNote>(Yaghmour & Mahmoud, 2013).lesser the size , larger the surface of NPs and have more absorption ability is one of the key point for the researches to work on nanotechnology. Nanotechnology is a science for the manipulation of matter at the nano level to form new materials and products. A naonometer is one billionth of a meter. Nanotechology is the attractive for the researches because of some unique properties of nano particles.i.e,. small size, incredible surface area, light weight, strong. ADDIN EN.CITE <EndNote><Cite><Author>Vance</Author><Year>2015</Year><RecNum>5</RecNum><DisplayText>(Vance et al., 2015)</DisplayText><record><rec-number>5</rec-number><foreign-keys><key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”>5</key></foreign-keys><ref-type name=”Journal Article”>17</ref-type><contributors><authors><author>Vance, Marina E</author><author>Kuiken, Todd</author><author>Vejerano, Eric P</author><author>McGinnis, Sean P</author><author>Hochella Jr, Michael F</author><author>Rejeski, David</author><author>Hull, Matthew S</author></authors></contributors><titles><title>Nanotechnology in the real world: Redeveloping the nanomaterial consumer products inventory</title><secondary-title>Beilstein journal of nanotechnology</secondary-title></titles><periodical><full-title>Beilstein journal of nanotechnology</full-title></periodical><pages>1769</pages><volume>6</volume><dates><year>2015</year></dates><urls></urls></record></Cite></EndNote>(Vance et al., 2015).

The first ever concept was presented in 1959 by the famous professor of Dr. Richard Feynman in the lecture of “there’s plenty of rooms at the bottom.” And the noble prize was awarded in 1965. The word nano technology had been coined by Norio Taniguchi in 1974. Dr. Eric Drexler gives the idea of molecular machinery manufacturing and computation in 1980’s. In 1960 feynman presented his visionary talk about nano tachnologies . In 1950 and 1960 on small metal particles there was experimental activity . at that time it was not called nano technology. In 1956, observation of porous silicon was first reported by Ulhi. ADDIN EN.CITE <EndNote><Cite><Author>Poole Jr</Author><Year>2003</Year><RecNum>7</RecNum><DisplayText>(Poole Jr &amp; Owens, 2003)</DisplayText><record><rec-number>7</rec-number><foreign-keys><key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”>7</key></foreign-keys><ref-type name=”Book”>6</ref-type><contributors><authors><author>Poole Jr, Charles P</author><author>Owens, Frank J</author></authors></contributors><titles><title>Introduction to nanotechnology</title></titles><dates><year>2003</year></dates><publisher>John Wiley &amp; Sons</publisher><isbn>0471079359</isbn><urls></urls></record></Cite></EndNote>(Poole Jr & Owens, 2003). 1000 years ago different size nano particles are used for produces strained glass windows. 2000 years ago sulphide nano crystals used by the greek and roman for the dye of the hair. In 4th century The glass contains gold silver alloyed nano particles which are distributed in such a way to make the glass look green in reflected lightbut, when light passes through the cup, it reveals a brilliant red.
A newely reported statement about the nanotechnology is ” nano science and technology will change the nature of almost every human made object in the next century ADDIN EN.CITE ;EndNote;;Cite;;Author;Klabunde;/Author;;Year;2001;/Year;;RecNum;8;/RecNum;;DisplayText;(Klabunde, 2001);/DisplayText;;record;;rec-number;8;/rec-number;;foreign-keys;;key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”;8;/key;;/foreign-keys;;ref-type name=”Journal Article”;17;/ref-type;;contributors;;authors;;author;Klabunde, Kenneth J;/author;;/authors;;/contributors;;titles;;title;Introduction to nanotechnology;/title;;secondary-title;Nanoscale Materials in Chemistry;/secondary-title;;/titles;;periodical;;full-title;Nanoscale Materials in Chemistry;/full-title;;/periodical;;pages;1-13;/pages;;dates;;year;2001;/year;;/dates;;urls;;/urls;;/record;;/Cite;;/EndNote;(Klabunde, 2001). Nano technology involves the following three attributes 1. research development and technology development at the atomic , moleculer , macro moleculer levels in the length scale of less then 100 nm. 2. Formation and use of structurs , systems , products and devices that have novel functions and the properties because of their small size. 3. An ability to control or manipulate on the atomic or the nano level. ADDIN EN.CITE ;EndNote;;Cite;;Author;Romig Jr;/Author;;Year;2007;/Year;;RecNum;10;/RecNum;;DisplayText;(Romig Jr et al., 2007);/DisplayText;;record;;rec-number;10;/rec-number;;foreign-keys;;key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”;10;/key;;/foreign-keys;;ref-type name=”Journal Article”;17;/ref-type;;contributors;;authors;;author;Romig Jr, AD;/author;;author;Baker, Arnold B;/author;;author;Johannes, Justine;/author;;author;Zipperian, Thomas;/author;;author;Eijkel, Kees;/author;;author;Kirchhoff, Bruce;/author;;author;Mani, HS;/author;;author;Rao, CNR;/author;;author;Walsh, Steven;/author;;/authors;;/contributors;;titles;;title;An introduction to nanotechnology policy: Opportunities and constraints for emerging and established economies;/title;;secondary-title;Technological Forecasting and Social Change;/secondary-title;;/titles;;periodical;;full-title;Technological Forecasting and Social Change;/full-title;;/periodical;;pages;1634-1642;/pages;;volume;74;/volume;;number;9;/number;;dates;;year;2007;/year;;/dates;;isbn;0040-1625;/isbn;;urls;;/urls;;/record;;/Cite;;/EndNote;(Romig Jr et al., 2007). The main difference between nanoscience and nano technology is that i.e., nanoscince is related to the phenomena and properties of materials that occurs at small length scale on the scale of atoms and molecules. However nano technology is the application of nano scale science engineering and technology for the productions of novel materials and novel devices such as materials such as material for biological and medical applications. Two techniques are used in nano technology i.e., bottom-up technique and top down technique. ADDIN EN.CITE ;EndNote;;Cite;;Author;Romig Jr;/Author;;Year;2007;/Year;;RecNum;11;/RecNum;;DisplayText;(Romig Jr, et al., 2007);/DisplayText;;record;;rec-number;11;/rec-number;;foreign-keys;;key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”;11;/key;;/foreign-keys;;ref-type name=”Journal Article”;17;/ref-type;;contributors;;authors;;author;Romig Jr, AD;/author;;author;Baker, Arnold B;/author;;author;Johannes, Justine;/author;;author;Zipperian, Thomas;/author;;author;Eijkel, Kees;/author;;author;Kirchhoff, Bruce;/author;;author;Mani, HS;/author;;author;Rao, CNR;/author;;author;Walsh, Steven;/author;;/authors;;/contributors;;titles;;title;An introduction to nanotechnology policy: Opportunities and constraints for emerging and established economies;/title;;secondary-title;Technological Forecasting and Social Change;/secondary-title;;/titles;;periodical;;full-title;Technological Forecasting and Social Change;/full-title;;/periodical;;pages;1634-1642;/pages;;volume;74;/volume;;number;9;/number;;dates;;year;2007;/year;;/dates;;isbn;0040-1625;/isbn;;urls;;/urls;;/record;;/Cite;;/EndNote;(Romig Jr, et al., 2007).

Top-down technique: Creating nano scale materials by physically and chemically breaking down larger materials.
Some of the following method of top down method:
Photo lithography:
photolithography uses light to expose a layer of radiation sensitive through mask polymer (photoresist).(a) prepare wafer (b) apply photo resist (c) Alldn photomask (d) expose to UV light (e) develop and remove photoresist exposed to UV light (f) Etch exposed oxide (g) remove remaining photoresist.
Scanning lithography:
Energetic particles such as electrons and ions can be used to pattern appropriate resist films leading to features with nanometer resolution. Recently established technology uses nano meter scanning probes for patterning resist. Films and is therefore reffered to as scanning probe lithography.

Electron beam liyhography:
Electron beam lithography or electron beam direct write lithography scans a focused beam of electrons on a surface covered with an electron sensitive film or resist to draw custom shapes. By changing the solubility of the resist and subsequent selective removal of material by immersion in a solvent sub 10 nm resolutions have been achieved.

Bottom up technique:
Assembling nano materials atom-by-atom or molecule-by-molecule.

Some of the following method for preparation of nanoparticle.

Chemical vapour deposition:
Precursor gases are delieverd into the reaction chamber at approximately ambient temperatures. As they pass over and come into contact with a heated substrate, they react or decompose forming a solid phase which and are deposited on to the substrate. The substrate temperature is crirical and can influence what reactions will take place.

Sol-gel method is also a method of bottom up technique.

Also there are thee distinct of aspect of nanotachnology which is termed as direct, indirect and conceptual.

Direct:Application of novel, nano engineered artifacts for example electronic and photonic circuit.

Indirect:Progressive miniaturization of existing technologies for example nano bots, cosmetics.

Conceptual:All materials and processes are considerd from a moleculer viewpoint for example universal manufacturing model.

Nano technology application in medicine( for example bio technology) in energy (for example re newable energy) electronics and information technology.

In nano technology nano materials can be formed by chemical method also from the green method. Chemical method have toxic and harmfull effects on environment. Whereas green method have no harmfull effect on environment.green method is non toxic and environmental friendly method.green method is mostly preferable beacause this method is cheaper and can be easily handle. Green method using biological routes and plants extracts for the synthesis of nano particles. these plants are easily available and and the nano particles can be synthesized under the normal circumstances.
RESEARCH OBJECTIVES
My research objectives are as follows:
? Using the field of nanotechnology, synthesis of nano particles.

? Chractrization of nanoparticles.

? Effect of prepared nano particles on the seed germination.
? Effect on Growth rate
? Check the anti microbial effect of seeds
? Anti fungal effect of seeds
LITERATURE REVIEW
Silver NPs were synthesized from silver nitrate thorough a simple green route using the Latex of Jatropha Curcas. NPs were chractrized with the help of HRTEM, X ray diffraction and ultra vilot visible absorption spectroscopy. ADDIN EN.CITE ;EndNote;;Cite;;Author;Bar;/Author;;Year;2009;/Year;;RecNum;12;/RecNum;;DisplayText;(Bar, Bhui, Sahoo, Sarkar, De, et al., 2009);/DisplayText;;record;;rec-number;12;/rec-number;;foreign-keys;;key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”;12;/key;;/foreign-keys;;ref-type name=”Journal Article”;17;/ref-type;;contributors;;authors;;author;Bar, Harekrishna;/author;;author;Bhui, Dipak Kr;/author;;author;Sahoo, Gobinda P;/author;;author;Sarkar, Priyanka;/author;;author;De, Sankar P;/author;;author;Misra, Ajay;/author;;/authors;;/contributors;;titles;;title;Green synthesis of silver nanoparticles using latex of Jatropha curcas;/title;;secondary-title;Colloids and surfaces A: Physicochemical and engineering aspects;/secondary-title;;/titles;;periodical;;full-title;Colloids and surfaces A: Physicochemical and engineering aspects;/full-title;;/periodical;;pages;134-139;/pages;;volume;339;/volume;;number;1-3;/number;;dates;;year;2009;/year;;/dates;;isbn;0927-7757;/isbn;;urls;;/urls;;/record;;/Cite;;/EndNote;(Bar, Bhui, Sahoo, Sarkar, De, et al., 2009). Synthesis of silver nano particles also reported using aqeous seed extract of jatropha curcas, Using the different concentrations of silver nitrate. Silver nano particles were chractrized by using three techniques i.e., (a) HRTEM, Xray diffraction, and UV visible spectroscopy. ADDIN EN.CITE ;EndNote;;Cite;;Author;Bar;/Author;;Year;2009;/Year;;RecNum;13;/RecNum;;DisplayText;(Bar, Bhui, Sahoo, Sarkar, Pyne, et al., 2009);/DisplayText;;record;;rec-number;13;/rec-number;;foreign-keys;;key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”;13;/key;;/foreign-keys;;ref-type name=”Journal Article”;17;/ref-type;;contributors;;authors;;author;Bar, Harekrishna;/author;;author;Bhui, Dipak Kr;/author;;author;Sahoo, Gobinda P;/author;;author;Sarkar, Priyanka;/author;;author;Pyne, Santanu;/author;;author;Misra, Ajay;/author;;/authors;;/contributors;;titles;;title;Green synthesis of silver nanoparticles using seed extract of Jatropha curcas;/title;;secondary-title;Colloids and surfaces A: Physicochemical and engineering aspects;/secondary-title;;/titles;;periodical;;full-title;Colloids and surfaces A: Physicochemical and engineering aspects;/full-title;;/periodical;;pages;212-216;/pages;;volume;348;/volume;;number;1-3;/number;;dates;;year;2009;/year;;/dates;;isbn;0927-7757;/isbn;;urls;;/urls;;/record;;/Cite;;/EndNote;(Bar, Bhui, Sahoo, Sarkar, Pyne, et al., 2009). Synthesis of silver nano particles reported using water extract of Terminalia chebula fruit under specific conditions. Formation of nano particles charactrized using FTIR, HRTEM. ADDIN EN.CITE ;EndNote;;Cite;;Author;Edison;/Author;;Year;2012;/Year;;RecNum;14;/RecNum;;DisplayText;(Edison ;amp; Sethuraman, 2012);/DisplayText;;record;;rec-number;14;/rec-number;;foreign-keys;;key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”;14;/key;;/foreign-keys;;ref-type name=”Journal Article”;17;/ref-type;;contributors;;authors;;author;Edison, T Jebakumar Immanuel;/author;;author;Sethuraman, MG;/author;;/authors;;/contributors;;titles;;title;Instant green synthesis of silver nanoparticles using Terminalia chebula fruit extract and evaluation of their catalytic activity on reduction of methylene blue;/title;;secondary-title;Process Biochemistry;/secondary-title;;/titles;;periodical;;full-title;Process Biochemistry;/full-title;;/periodical;;pages;1351-1357;/pages;;volume;47;/volume;;number;9;/number;;dates;;year;2012;/year;;/dates;;isbn;1359-5113;/isbn;;urls;;/urls;;/record;;/Cite;;/EndNote;(Edison ; Sethuraman, 2012). Green synthesis of nano particles reported using ascorbic acid and chitosan. ADDIN EN.CITE ;EndNote;;Cite;;Author;Zain;/Author;;Year;2014;/Year;;RecNum;15;/RecNum;;DisplayText;(Zain, Stapley, ;amp; Shama, 2014);/DisplayText;;record;;rec-number;15;/rec-number;;foreign-keys;;key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”;15;/key;;/foreign-keys;;ref-type name=”Journal Article”;17;/ref-type;;contributors;;authors;;author;Zain, N Mat;/author;;author;Stapley, AGF;/author;;author;Shama, Gilbert;/author;;/authors;;/contributors;;titles;;title;Green synthesis of silver and copper nanoparticles using Ascorbic acid and Chitosan for antimicrobial applications;/title;;secondary-title;Carbohydrate polymers;/secondary-title;;/titles;;periodical;;full-title;Carbohydrate polymers;/full-title;;/periodical;;pages;195-202;/pages;;volume;112;/volume;;dates;;year;2014;/year;;/dates;;isbn;0144-8617;/isbn;;urls;;/urls;;/record;;/Cite;;/EndNote;(Zain, Stapley, ; Shama, 2014). Synthesis of silver nano particles reported by using Penicillium Purpurogenum also the anti microbial activity of the Ag NPs and PH effect. ADDIN EN.CITE ;EndNote;;Cite;;Author;Nayak;/Author;;Year;2011;/Year;;RecNum;16;/RecNum;;DisplayText;(Nayak et al., 2011);/DisplayText;;record;;rec-number;16;/rec-number;;foreign-keys;;key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”;16;/key;;/foreign-keys;;ref-type name=”Journal Article”;17;/ref-type;;contributors;;authors;;author;Nayak, Rati Ranjan;/author;;author;Pradhan, Nilotpala;/author;;author;Behera, Debadhyan;/author;;author;Pradhan, Kshyama Madhusikta;/author;;author;Mishra, Srabani;/author;;author;Sukla, Lala Behari;/author;;author;Mishra, Barada Kanta;/author;;/authors;;/contributors;;titles;;title;Green synthesis of silver nanoparticle by Penicillium purpurogenum NPMF: the process and optimization;/title;;secondary-title;Journal of Nanoparticle Research;/secondary-title;;/titles;;periodical;;full-title;Journal of Nanoparticle Research;/full-title;;/periodical;;pages;3129-3137;/pages;;volume;13;/volume;;number;8;/number;;dates;;year;2011;/year;;/dates;;isbn;1388-0764;/isbn;;urls;;/urls;;/record;;/Cite;;/EndNote;(Nayak et al., 2011).

Formation of silver nano particles using green method were reported with the aqeous extract of Gloriosa Superba Linn i.e. Glory Lilly. Silver nano particles were characterized by UV-visible, Fourier Transform Infrared, X Ray Diffraction, Transmission Electron Microscopy, Scanning Electron Microscopy, Energy Dispersive X-Ray, Photoluminescence. The synthesized nano particles are observed have a good catalytic activity. ADDIN EN.CITE ;EndNote;;Cite;;Author;Ashokkumar;/Author;;Year;2013;/Year;;RecNum;17;/RecNum;;DisplayText;(Ashokkumar, Ravi, ;amp; Velmurugan, 2013);/DisplayText;;record;;rec-number;17;/rec-number;;foreign-keys;;key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”;17;/key;;/foreign-keys;;ref-type name=”Generic”;13;/ref-type;;contributors;;authors;;author;Ashokkumar, S;/author;;author;Ravi, S;/author;;author;Velmurugan, S;/author;;/authors;;/contributors;;titles;;title;RETRACTED: Green synthesis of silver nanoparticles from Gloriosa superba L. leaf extract and their catalytic activity;/title;;/titles;;dates;;year;2013;/year;;/dates;;publisher;Elsevier;/publisher;;isbn;1386-1425;/isbn;;urls;;/urls;;/record;;/Cite;;/EndNote;(Ashokkumar, Ravi, ; Velmurugan, 2013). Synthesis of Ag NPs by microwave irradiation using the leaf extract of Biophytum Sensitivum. Analyzed Nanoparticles were chractrized by using the techniques Ultra violot visible, Forier Transform Infrared, X-Ray Diffraction Technique and HRTEM. ADDIN EN.CITE ;EndNote;;Cite;;Author;Joseph;/Author;;Year;2015;/Year;;RecNum;18;/RecNum;;DisplayText;(Joseph ;amp; Mathew, 2015);/DisplayText;;record;;rec-number;18;/rec-number;;foreign-keys;;key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”;18;/key;;/foreign-keys;;ref-type name=”Journal Article”;17;/ref-type;;contributors;;authors;;author;Joseph, Siby;/author;;author;Mathew, Beena;/author;;/authors;;/contributors;;titles;;title;Microwave-assisted green synthesis of silver nanoparticles and the study on catalytic activity in the degradation of dyes;/title;;secondary-title;Journal of Molecular Liquids;/secondary-title;;/titles;;periodical;;full-title;Journal of Molecular Liquids;/full-title;;/periodical;;pages;184-191;/pages;;volume;204;/volume;;dates;;year;2015;/year;;/dates;;isbn;0167-7322;/isbn;;urls;;/urls;;/record;;/Cite;;/EndNote;(Joseph ; Mathew, 2015). Silver nano particles can be synthesized using plants extracts for example Azadirachta Indica ( commonly named as NEEM ) Ingiber Officinale( commonly named as Ginger). Reported synthesis by the process of biochemical reaction between silver salt solution with seed extract of Coriandrum Sativum with out using any external energy. The formation of nano particles characterized by using different techniques like Transmission Electron Microscopy. ADDIN EN.CITE ;EndNote;;Cite;;Author;Nazeruddin;/Author;;Year;2014;/Year;;RecNum;19;/RecNum;;DisplayText;(Nazeruddin et al., 2014);/DisplayText;;record;;rec-number;19;/rec-number;;foreign-keys;;key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”;19;/key;;/foreign-keys;;ref-type name=”Journal Article”;17;/ref-type;;contributors;;authors;;author;Nazeruddin, GM;/author;;author;Prasad, NR;/author;;author;Prasad, SR;/author;;author;Shaikh, YI;/author;;author;Waghmare, SR;/author;;author;Adhyapak, Parag;/author;;/authors;;/contributors;;titles;;title;Coriandrum sativum seed extract assisted in situ green synthesis of silver nanoparticle and its anti-microbial activity;/title;;secondary-title;Industrial Crops and Products;/secondary-title;;/titles;;periodical;;full-title;Industrial Crops and Products;/full-title;;/periodical;;pages;212-216;/pages;;volume;60;/volume;;dates;;year;2014;/year;;/dates;;isbn;0926-6690;/isbn;;urls;;/urls;;/record;;/Cite;;/EndNote;(Nazeruddin et al., 2014).
Green synthesis of nano particles were reported by using European Black ElderBerry fruit extracts, these particles characterized by UVB irradiation. ADDIN EN.CITE ;EndNote;;Cite;;Author;David;/Author;;Year;2014;/Year;;RecNum;20;/RecNum;;DisplayText;(David et al., 2014);/DisplayText;;record;;rec-number;20;/rec-number;;foreign-keys;;key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”;20;/key;;/foreign-keys;;ref-type name=”Journal Article”;17;/ref-type;;contributors;;authors;;author;David, Luminita;/author;;author;Moldovan, Bianca;/author;;author;Vulcu, Adriana;/author;;author;Olenic, Liliana;/author;;author;Perde-Schrepler, Maria;/author;;author;Fischer-Fodor, Eva;/author;;author;Florea, Adrian;/author;;author;Crisan, Maria;/author;;author;Chiorean, Ioana;/author;;author;Clichici, Simona;/author;;/authors;;/contributors;;titles;;title;Green synthesis, characterization and anti-inflammatory activity of silver nanoparticles using European black elderberry fruits extract;/title;;secondary-title;Colloids and Surfaces B: Biointerfaces;/secondary-title;;/titles;;periodical;;full-title;Colloids and Surfaces B: Biointerfaces;/full-title;;/periodical;;pages;767-777;/pages;;volume;122;/volume;;dates;;year;2014;/year;;/dates;;isbn;0927-7765;/isbn;;urls;;/urls;;/record;;/Cite;;/EndNote;(David et al., 2014). Synthesis of silver nano particles reported from Carica Papaya Leaf extract and their shape and size were characterized by using SEM (scanning electron microscopy, TEM (transmission electron microscopy), FTIR (Fourier Transform Infrared Spectroscopy) EDS and EDX (Energy Dispersive X-Ray Spectroscopy and XRD ( X-Ray Diffraction Spectroscopy ). ADDIN EN.CITE ;EndNote;;Cite;;Author;Banala;/Author;;Year;2015;/Year;;RecNum;21;/RecNum;;DisplayText;(Banala, Nagati, ;amp; Karnati, 2015);/DisplayText;;record;;rec-number;21;/rec-number;;foreign-keys;;key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”;21;/key;;/foreign-keys;;ref-type name=”Journal Article”;17;/ref-type;;contributors;;authors;;author;Banala, Rajkiran Reddy;/author;;author;Nagati, Veera Babu;/author;;author;Karnati, Pratap Reddy;/author;;/authors;;/contributors;;titles;;title;Green synthesis and characterization of Carica papaya leaf extract coated silver nanoparticles through X-ray diffraction, electron microscopy and evaluation of bactericidal properties;/title;;secondary-title;Saudi journal of biological sciences;/secondary-title;;/titles;;periodical;;full-title;Saudi journal of biological sciences;/full-title;;/periodical;;pages;637-644;/pages;;volume;22;/volume;;number;5;/number;;dates;;year;2015;/year;;/dates;;isbn;1319-562X;/isbn;;urls;;/urls;;/record;;/Cite;;/EndNote;(Banala, Nagati, ; Karnati, 2015). Spherical Ag nano particles were synthesized by using Aloe Vera Leaf extract , and characterized by using the UV-VIS-NIR absorption spectroscopy and TEM (transmission Electron Microscopy). ADDIN EN.CITE ;EndNote;;Cite;;Author;Chandran;/Author;;Year;2006;/Year;;RecNum;22;/RecNum;;DisplayText;(Chandran, Chaudhary, Pasricha, Ahmad, ;amp; Sastry, 2006);/DisplayText;;record;;rec-number;22;/rec-number;;foreign-keys;;key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”;22;/key;;/foreign-keys;;ref-type name=”Journal Article”;17;/ref-type;;contributors;;authors;;author;Chandran, S Prathap;/author;;author;Chaudhary, Minakshi;/author;;author;Pasricha, Renu;/author;;author;Ahmad, Absar;/author;;author;Sastry, Murali;/author;;/authors;;/contributors;;titles;;title;Synthesis of gold nanotriangles and silver nanoparticles using Aloevera plant extract;/title;;secondary-title;Biotechnology progress;/secondary-title;;/titles;;periodical;;full-title;Biotechnology progress;/full-title;;/periodical;;pages;577-583;/pages;;volume;22;/volume;;number;2;/number;;dates;;year;2006;/year;;/dates;;isbn;8756-7938;/isbn;;urls;;/urls;;/record;;/Cite;;/EndNote;(Chandran, Chaudhary, Pasricha, Ahmad, ; Sastry, 2006). Reported green synthesis of silver nano particles using one mM silver nitrate through the plants extracts (including leaves and fruits) of Securinega Leucopyrus mull. These nanoparticles were characterized by ultra violot visible absorption spectroscopy, Fourier transform infra red, transmission electron microscopy and scanning electron microscopy. ADDIN EN.CITE ;EndNote;;Cite;;Author;Donda;/Author;;Year;2013;/Year;;RecNum;23;/RecNum;;DisplayText;(Donda et al., 2013);/DisplayText;;record;;rec-number;23;/rec-number;;foreign-keys;;key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”;23;/key;;/foreign-keys;;ref-type name=”Journal Article”;17;/ref-type;;contributors;;authors;;author;Donda, Manisha R;/author;;author;Kudle, Karunakar Rao;/author;;author;Alwala, Jahnavi;/author;;author;Miryala, Anila;/author;;author;Sreedhar, B;/author;;author;Rudra, MP Pratap;/author;;/authors;;/contributors;;titles;;title;Synthesis of silver nanoparticles using extracts of Securinega leucopyrus and evaluation of its antibacterial activity;/title;;secondary-title;Int J Curr Sci;/secondary-title;;/titles;;periodical;;full-title;Int J Curr Sci;/full-title;;/periodical;;pages;1-8;/pages;;volume;7;/volume;;dates;;year;2013;/year;;/dates;;urls;;/urls;;/record;;/Cite;;/EndNote;(Donda et al., 2013). Green synthesis of silver nano particles were reported by using Phoma Glomerata and the formed nano particles were characterized by UV visible spectro photo meter, and Fourier transform infrared spectroscopy, and for the size detection of silver nano particles scanning electron microscopy were used. ADDIN EN.CITE ;EndNote;;Cite;;Author;Birla;/Author;;Year;2009;/Year;;RecNum;24;/RecNum;;DisplayText;(Birla et al., 2009);/DisplayText;;record;;rec-number;24;/rec-number;;foreign-keys;;key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”;24;/key;;/foreign-keys;;ref-type name=”Journal Article”;17;/ref-type;;contributors;;authors;;author;Birla, SS;/author;;author;Tiwari, VV;/author;;author;Gade, AK;/author;;author;Ingle, AP;/author;;author;Yadav, AP;/author;;author;Rai, MK;/author;;/authors;;/contributors;;titles;;title;Fabrication of silver nanoparticles by Phoma glomerata and its combined effect against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus;/title;;secondary-title;Letters in Applied Microbiology;/secondary-title;;/titles;;periodical;;full-title;Letters in Applied Microbiology;/full-title;;/periodical;;pages;173-179;/pages;;volume;48;/volume;;number;2;/number;;dates;;year;2009;/year;;/dates;;isbn;1472-765X;/isbn;;urls;;/urls;;/record;;/Cite;;/EndNote;(Birla et al., 2009).
Synthesis of zinc sulphide also reported by green method using 0.3 percent Latex solution prepared from Jatropha Curcas, prepared zinc sulphide nano particles were characterized by XRD, (X-ray diffraction), SAED (selected area electron diffraction), TEM (transmission electron microscopy), EDX (energy dispersive analysis of x-rays), UV visible optical absorption and photo luminescence technique. ADDIN EN.CITE ;EndNote;;Cite;;Author;Hudlikar;/Author;;Year;2012;/Year;;RecNum;25;/RecNum;;DisplayText;(Hudlikar, Joglekar, Dhaygude, ;amp; Kodam, 2012);/DisplayText;;record;;rec-number;25;/rec-number;;foreign-keys;;key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”;25;/key;;/foreign-keys;;ref-type name=”Journal Article”;17;/ref-type;;contributors;;authors;;author;Hudlikar, Manish;/author;;author;Joglekar, Shreeram;/author;;author;Dhaygude, Mayur;/author;;author;Kodam, Kisan;/author;;/authors;;/contributors;;titles;;title;Latex-mediated synthesis of ZnS nanoparticles: green synthesis approach;/title;;secondary-title;Journal of Nanoparticle Research;/secondary-title;;/titles;;periodical;;full-title;Journal of Nanoparticle Research;/full-title;;/periodical;;pages;865;/pages;;volume;14;/volume;;number;5;/number;;dates;;year;2012;/year;;/dates;;isbn;1388-0764;/isbn;;urls;;/urls;;/record;;/Cite;;/EndNote;(Hudlikar, Joglekar, Dhaygude, ; Kodam, 2012). Reported preparation of zinc sulphide by the green method using the extract of Pleurotuss Ostreatu commonly named as mushroom, the analyzed nano particles were characterized by XRD ( X ray diffraction ), EDX (energy dispersive analysis of X-Rays), SEM (scanning electron microscopy), TEM (transmission electron microscopy), UV visible optical absorption, photo luminescence and FTIR (fourier transform infrared). ADDIN EN.CITE ;EndNote;;Cite;;Author;Senapati;/Author;;Year;2014;/Year;;RecNum;26;/RecNum;;DisplayText;(Senapati ;amp; Sarkar, 2014);/DisplayText;;record;;rec-number;26;/rec-number;;foreign-keys;;key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”;26;/key;;/foreign-keys;;ref-type name=”Journal Article”;17;/ref-type;;contributors;;authors;;author;Senapati, US;/author;;author;Sarkar, D;/author;;/authors;;/contributors;;titles;;title;Characterization of biosynthesized zinc sulphide nanoparticles using edible mushroom Pleurotuss ostreatu;/title;;secondary-title;Indian Journal of Physics;/secondary-title;;/titles;;periodical;;full-title;Indian Journal of Physics;/full-title;;/periodical;;pages;557-562;/pages;;volume;88;/volume;;number;6;/number;;dates;;year;2014;/year;;/dates;;isbn;0973-1458;/isbn;;urls;;/urls;;/record;;/Cite;;/EndNote;(Senapati ; Sarkar, 2014). By Green method synthesis of zinc sulphide nano particles reported by using rhannolipids from pseudomonas aeruginosa BS01 as capping and stabilizing agent, NPs characterized by using techniques X ray diffraction spectroscopy, scanning electron microscopy, transmission electron microscopy, ultra violet irradiation. ADDIN EN.CITE ;EndNote;;Cite;;Author;Hazra;/Author;;Year;2013;/Year;;RecNum;27;/RecNum;;DisplayText;(Hazra, Kundu, Chaudhari, ;amp; Jana, 2013);/DisplayText;;record;;rec-number;27;/rec-number;;foreign-keys;;key app=”EN” db-id=”x5r9ftrfgvzav0etsepvtxfc0wpv0z9psfae”;27;/key;;/foreign-keys;;ref-type name=”Journal Article”;17;/ref-type;;contributors;;authors;;author;Hazra, Chinmay;/author;;author;Kundu, Debasree;/author;;author;Chaudhari, Ambalal;/author;;author;Jana, Tushar;/author;;/authors;;/contributors;;titles;;title;Biogenic synthesis, characterization, toxicity and photocatalysis of zinc sulfide nanoparticles using rhamnolipids from Pseudomonas aeruginosa BS01 as capping and stabilizing agent;/title;;secondary-title;Journal of Chemical Technology ;amp; Biotechnology;/secondary-title;;/titles;;periodical;;full-title;Journal of Chemical Technology ;amp; Biotechnology;/full-title;;/periodical;;pages;1039-1048;/pages;;volume;88;/volume;;number;6;/number;;dates;;year;2013;/year;;/dates;;isbn;0268-2575;/isbn;;urls;;/urls;;/record;;/Cite;;/EndNote;(Hazra, Kundu, Chaudhari, ; Jana, 2013).

MATERIAL AND METHODS
All experimental work will be performed in science Lab university of the Gujrat, Gujrat.

Materials and Method:
We use Green synthesis (Green method ) for the synthesis of metal sulphides i.e, silver sulphide nanoparticles and zinc sulphide particles. In this method we use plants extracts for the synthesis of nano particles, leaves and flowers etc. Green method is eco-friendly cheaper and non-toxic, also green method have no harmfull effect on invoironment.

For the synthesis of silver sulfide and zinc sulfide we will use Azadirachta Indica i.e, common name Neem and Zingiber officinale i.e, common name Ginger. We will take the leaves and then dry to make in the form of powder after that we will make nano particles from that powder. It is an cost effective method and have no toxic effect on surrounding environment.For the characterization of these synthesized nanoparticles we will use different techniques including:XRD, EDAX, SEM and TEM are used for the size shape and morphology of nano particles. then we will check the effect of these prepared nano particles on seed germination. This work will b performed in petri dishes with the varreying amount of these nano particles. And a software is used for the graphical work that is named as origin.

EXPECTED OUTCOMES
We will synthesize nanoparticles with more precise shape and size by green method. And by using that nanoparticles we will check the seed germination, germination rate will be increase we achieve better growth mean time. Using the different ratio of silver sulphide and zinc sulphide will give the different positive results in growth rate. They will increase the growth time, growth rate and ilimunates the toxic effects from the soil.
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